g actin pyrene Search Results


pyrene actin polymerization kit  (Cytoskeleton Inc)
95
Cytoskeleton Inc pyrene actin polymerization kit
Impact of TmeB on Arp2/3-mediated <t>actin</t> <t>polymerization.</t> (A) Coomassie stained preparations of the purified Actin, Arp2/3 complex, the Verprolin homology, Central, Acidic domain of N-WASP, and TmeB employed in the <t>pyrene</t> actin polymerization assay. Increase in actin polymerization after the addition of polymerization buffer at 300 s was measured as the arbitrary fluorescence intensity (arbitrary units [a.u.] over time [s]) with excitation and emission wavelengths of 365 and 407 nm respectively. (B) TmeB, Arp2/3 and VCA were added individually or in combination to monomeric pyrene-labeled actin and actin alone served as a negative control. (C) Like the assay shown in (A) but with 2-fold increases in the amount of pure TmeB included in mixtures. Maximal actin polymerization occurred in the presence of Arp2/3 and VCA and this was inhibited by the presence of TmeB.
Pyrene Actin Polymerization Kit, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/g+actin+pyrene/Actin+Polymerization+Biochem+Kit/pmc10360934-88-7-11
Average 95 stars, based on 1 article reviews
pyrene actin polymerization kit - by Bioz Stars, 2026-09
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pyrene  (Cytoskeleton Inc)
93
Cytoskeleton Inc pyrene
Impact of TmeB on Arp2/3-mediated <t>actin</t> <t>polymerization.</t> (A) Coomassie stained preparations of the purified Actin, Arp2/3 complex, the Verprolin homology, Central, Acidic domain of N-WASP, and TmeB employed in the <t>pyrene</t> actin polymerization assay. Increase in actin polymerization after the addition of polymerization buffer at 300 s was measured as the arbitrary fluorescence intensity (arbitrary units [a.u.] over time [s]) with excitation and emission wavelengths of 365 and 407 nm respectively. (B) TmeB, Arp2/3 and VCA were added individually or in combination to monomeric pyrene-labeled actin and actin alone served as a negative control. (C) Like the assay shown in (A) but with 2-fold increases in the amount of pure TmeB included in mixtures. Maximal actin polymerization occurred in the presence of Arp2/3 and VCA and this was inhibited by the presence of TmeB.
Pyrene, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/g+actin+pyrene/Pyrene+Labeled+Actin+Protein/pmc03421996-114-0-2
Average 93 stars, based on 1 article reviews
pyrene - by Bioz Stars, 2026-09
93/100 stars
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ar05 pyrene labeled actin g actin pyrene hypermol  (Cytoskeleton Inc)
95
Cytoskeleton Inc ar05 pyrene labeled actin g actin pyrene hypermol
Impact of TmeB on Arp2/3-mediated <t>actin</t> <t>polymerization.</t> (A) Coomassie stained preparations of the purified Actin, Arp2/3 complex, the Verprolin homology, Central, Acidic domain of N-WASP, and TmeB employed in the <t>pyrene</t> actin polymerization assay. Increase in actin polymerization after the addition of polymerization buffer at 300 s was measured as the arbitrary fluorescence intensity (arbitrary units [a.u.] over time [s]) with excitation and emission wavelengths of 365 and 407 nm respectively. (B) TmeB, Arp2/3 and VCA were added individually or in combination to monomeric pyrene-labeled actin and actin alone served as a negative control. (C) Like the assay shown in (A) but with 2-fold increases in the amount of pure TmeB included in mixtures. Maximal actin polymerization occurred in the presence of Arp2/3 and VCA and this was inhibited by the presence of TmeB.
Ar05 Pyrene Labeled Actin G Actin Pyrene Hypermol, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/g+actin+pyrene/Actin+protein+rhodamine+rabbit+skeletal+muscle/pmc10976963__nn3c12265_si_001-115-22-20
Average 95 stars, based on 1 article reviews
ar05 pyrene labeled actin g actin pyrene hypermol - by Bioz Stars, 2026-09
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g actin  (Cytoskeleton Inc)
95
Cytoskeleton Inc g actin
Impact of TmeB on Arp2/3-mediated <t>actin</t> <t>polymerization.</t> (A) Coomassie stained preparations of the purified Actin, Arp2/3 complex, the Verprolin homology, Central, Acidic domain of N-WASP, and TmeB employed in the <t>pyrene</t> actin polymerization assay. Increase in actin polymerization after the addition of polymerization buffer at 300 s was measured as the arbitrary fluorescence intensity (arbitrary units [a.u.] over time [s]) with excitation and emission wavelengths of 365 and 407 nm respectively. (B) TmeB, Arp2/3 and VCA were added individually or in combination to monomeric pyrene-labeled actin and actin alone served as a negative control. (C) Like the assay shown in (A) but with 2-fold increases in the amount of pure TmeB included in mixtures. Maximal actin polymerization occurred in the presence of Arp2/3 and VCA and this was inhibited by the presence of TmeB.
G Actin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/g+actin+pyrene/Actin+protein+-+pyrene+rabbit+skeletal+muscle/10__1074_slash_jbc__m301997200-81-3-11
Average 95 stars, based on 1 article reviews
g actin - by Bioz Stars, 2026-09
95/100 stars
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pyrene iodoacetamide labelled actin pi actin  (Cytoskeleton Inc)
94
Cytoskeleton Inc pyrene iodoacetamide labelled actin pi actin
Impact of TmeB on Arp2/3-mediated <t>actin</t> <t>polymerization.</t> (A) Coomassie stained preparations of the purified Actin, Arp2/3 complex, the Verprolin homology, Central, Acidic domain of N-WASP, and TmeB employed in the <t>pyrene</t> actin polymerization assay. Increase in actin polymerization after the addition of polymerization buffer at 300 s was measured as the arbitrary fluorescence intensity (arbitrary units [a.u.] over time [s]) with excitation and emission wavelengths of 365 and 407 nm respectively. (B) TmeB, Arp2/3 and VCA were added individually or in combination to monomeric pyrene-labeled actin and actin alone served as a negative control. (C) Like the assay shown in (A) but with 2-fold increases in the amount of pure TmeB included in mixtures. Maximal actin polymerization occurred in the presence of Arp2/3 and VCA and this was inhibited by the presence of TmeB.
Pyrene Iodoacetamide Labelled Actin Pi Actin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/g+actin+pyrene/Actin+protein+pure+rabbit+skeletal+muscle/pm30040977-88-6-14
Average 94 stars, based on 1 article reviews
pyrene iodoacetamide labelled actin pi actin - by Bioz Stars, 2026-09
94/100 stars
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glass container  (Corning Life Sciences)
90
Corning Life Sciences glass container
Impact of TmeB on Arp2/3-mediated <t>actin</t> <t>polymerization.</t> (A) Coomassie stained preparations of the purified Actin, Arp2/3 complex, the Verprolin homology, Central, Acidic domain of N-WASP, and TmeB employed in the <t>pyrene</t> actin polymerization assay. Increase in actin polymerization after the addition of polymerization buffer at 300 s was measured as the arbitrary fluorescence intensity (arbitrary units [a.u.] over time [s]) with excitation and emission wavelengths of 365 and 407 nm respectively. (B) TmeB, Arp2/3 and VCA were added individually or in combination to monomeric pyrene-labeled actin and actin alone served as a negative control. (C) Like the assay shown in (A) but with 2-fold increases in the amount of pure TmeB included in mixtures. Maximal actin polymerization occurred in the presence of Arp2/3 and VCA and this was inhibited by the presence of TmeB.
Glass Container, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/g+actin+pyrene/glass+container/10__1590_slash_s0104___66322008000100015-58-15-14
Average 90 stars, based on 1 article reviews
glass container - by Bioz Stars, 2026-09
90/100 stars
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pyrene-actin fluorescence  (SCHOTT)
90
SCHOTT pyrene-actin fluorescence
Impact of TmeB on Arp2/3-mediated <t>actin</t> <t>polymerization.</t> (A) Coomassie stained preparations of the purified Actin, Arp2/3 complex, the Verprolin homology, Central, Acidic domain of N-WASP, and TmeB employed in the <t>pyrene</t> actin polymerization assay. Increase in actin polymerization after the addition of polymerization buffer at 300 s was measured as the arbitrary fluorescence intensity (arbitrary units [a.u.] over time [s]) with excitation and emission wavelengths of 365 and 407 nm respectively. (B) TmeB, Arp2/3 and VCA were added individually or in combination to monomeric pyrene-labeled actin and actin alone served as a negative control. (C) Like the assay shown in (A) but with 2-fold increases in the amount of pure TmeB included in mixtures. Maximal actin polymerization occurred in the presence of Arp2/3 and VCA and this was inhibited by the presence of TmeB.
Pyrene Actin Fluorescence, supplied by SCHOTT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/g+actin+pyrene/pyrene+actin+fluorescence/pmc04110310-459-26-38
Average 90 stars, based on 1 article reviews
pyrene-actin fluorescence - by Bioz Stars, 2026-09
90/100 stars
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pyrene-labeled actin  (Matreya LLC)
90
Matreya LLC pyrene-labeled actin
Impact of TmeB on Arp2/3-mediated <t>actin</t> <t>polymerization.</t> (A) Coomassie stained preparations of the purified Actin, Arp2/3 complex, the Verprolin homology, Central, Acidic domain of N-WASP, and TmeB employed in the <t>pyrene</t> actin polymerization assay. Increase in actin polymerization after the addition of polymerization buffer at 300 s was measured as the arbitrary fluorescence intensity (arbitrary units [a.u.] over time [s]) with excitation and emission wavelengths of 365 and 407 nm respectively. (B) TmeB, Arp2/3 and VCA were added individually or in combination to monomeric pyrene-labeled actin and actin alone served as a negative control. (C) Like the assay shown in (A) but with 2-fold increases in the amount of pure TmeB included in mixtures. Maximal actin polymerization occurred in the presence of Arp2/3 and VCA and this was inhibited by the presence of TmeB.
Pyrene Labeled Actin, supplied by Matreya LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/g+actin+pyrene/pyrene+labeled+actin/pm15664188-218-4-21
Average 90 stars, based on 1 article reviews
pyrene-labeled actin - by Bioz Stars, 2026-09
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rabbit skeletal muscle actin  (Cytoskeleton Inc)
95
Cytoskeleton Inc rabbit skeletal muscle actin
Impact of TmeB on Arp2/3-mediated <t>actin</t> <t>polymerization.</t> (A) Coomassie stained preparations of the purified Actin, Arp2/3 complex, the Verprolin homology, Central, Acidic domain of N-WASP, and TmeB employed in the <t>pyrene</t> actin polymerization assay. Increase in actin polymerization after the addition of polymerization buffer at 300 s was measured as the arbitrary fluorescence intensity (arbitrary units [a.u.] over time [s]) with excitation and emission wavelengths of 365 and 407 nm respectively. (B) TmeB, Arp2/3 and VCA were added individually or in combination to monomeric pyrene-labeled actin and actin alone served as a negative control. (C) Like the assay shown in (A) but with 2-fold increases in the amount of pure TmeB included in mixtures. Maximal actin polymerization occurred in the presence of Arp2/3 and VCA and this was inhibited by the presence of TmeB.
Rabbit Skeletal Muscle Actin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/g+actin+pyrene/Actin+protein+-+pre-formed+filaments+rabbit+skeletal+muscle/pmc05748978-247-6-12
Average 95 stars, based on 1 article reviews
rabbit skeletal muscle actin - by Bioz Stars, 2026-09
95/100 stars
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rabbit muscle pyrene actin  (Cytoskeleton Inc)
96
Cytoskeleton Inc rabbit muscle pyrene actin
Impact of TmeB on Arp2/3-mediated <t>actin</t> <t>polymerization.</t> (A) Coomassie stained preparations of the purified Actin, Arp2/3 complex, the Verprolin homology, Central, Acidic domain of N-WASP, and TmeB employed in the <t>pyrene</t> actin polymerization assay. Increase in actin polymerization after the addition of polymerization buffer at 300 s was measured as the arbitrary fluorescence intensity (arbitrary units [a.u.] over time [s]) with excitation and emission wavelengths of 365 and 407 nm respectively. (B) TmeB, Arp2/3 and VCA were added individually or in combination to monomeric pyrene-labeled actin and actin alone served as a negative control. (C) Like the assay shown in (A) but with 2-fold increases in the amount of pure TmeB included in mixtures. Maximal actin polymerization occurred in the presence of Arp2/3 and VCA and this was inhibited by the presence of TmeB.
Rabbit Muscle Pyrene Actin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/g+actin+pyrene/Actin+protein+-+rabbit+skeletal+muscle+%3E99%25+pure/10__1074_slash_jbc__m113__461277-95-10-14
Average 96 stars, based on 1 article reviews
rabbit muscle pyrene actin - by Bioz Stars, 2026-09
96/100 stars
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Image Search Results


Impact of TmeB on Arp2/3-mediated actin polymerization. (A) Coomassie stained preparations of the purified Actin, Arp2/3 complex, the Verprolin homology, Central, Acidic domain of N-WASP, and TmeB employed in the pyrene actin polymerization assay. Increase in actin polymerization after the addition of polymerization buffer at 300 s was measured as the arbitrary fluorescence intensity (arbitrary units [a.u.] over time [s]) with excitation and emission wavelengths of 365 and 407 nm respectively. (B) TmeB, Arp2/3 and VCA were added individually or in combination to monomeric pyrene-labeled actin and actin alone served as a negative control. (C) Like the assay shown in (A) but with 2-fold increases in the amount of pure TmeB included in mixtures. Maximal actin polymerization occurred in the presence of Arp2/3 and VCA and this was inhibited by the presence of TmeB.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Chlamydia trachomatis TmeB antagonizes actin polymerization via direct interference with Arp2/3 activity

doi: 10.3389/fcimb.2023.1232391

Figure Lengend Snippet: Impact of TmeB on Arp2/3-mediated actin polymerization. (A) Coomassie stained preparations of the purified Actin, Arp2/3 complex, the Verprolin homology, Central, Acidic domain of N-WASP, and TmeB employed in the pyrene actin polymerization assay. Increase in actin polymerization after the addition of polymerization buffer at 300 s was measured as the arbitrary fluorescence intensity (arbitrary units [a.u.] over time [s]) with excitation and emission wavelengths of 365 and 407 nm respectively. (B) TmeB, Arp2/3 and VCA were added individually or in combination to monomeric pyrene-labeled actin and actin alone served as a negative control. (C) Like the assay shown in (A) but with 2-fold increases in the amount of pure TmeB included in mixtures. Maximal actin polymerization occurred in the presence of Arp2/3 and VCA and this was inhibited by the presence of TmeB.

Article Snippet: Remaining components were from the commercially available pyrene actin polymerization kit (Cytoskeleton).

Techniques: Staining, Purification, Polymerization Assay, Fluorescence, Labeling, Negative Control

TmeB inhibition in the presence of TmeA-N-WASP or CK666. Actin polymerization was tested measured as the arbitrary fluorescence intensity (arbitrary units [a.u.] over time [s]) with excitation and emission wavelengths of 365 and 407 nm respectively. Polymerization buffer was added at 300 s. Monomeric pyrene-labeled actin and actin alone served as a negative control. (A) TmeA and N-WASP 151-501 were added individually or in combination with TmeB. (B) TmeB, Arp2/3 and VCA were added individually or in combination with 17 µM of the Arp2/3 inhibitor CK666.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Chlamydia trachomatis TmeB antagonizes actin polymerization via direct interference with Arp2/3 activity

doi: 10.3389/fcimb.2023.1232391

Figure Lengend Snippet: TmeB inhibition in the presence of TmeA-N-WASP or CK666. Actin polymerization was tested measured as the arbitrary fluorescence intensity (arbitrary units [a.u.] over time [s]) with excitation and emission wavelengths of 365 and 407 nm respectively. Polymerization buffer was added at 300 s. Monomeric pyrene-labeled actin and actin alone served as a negative control. (A) TmeA and N-WASP 151-501 were added individually or in combination with TmeB. (B) TmeB, Arp2/3 and VCA were added individually or in combination with 17 µM of the Arp2/3 inhibitor CK666.

Article Snippet: Remaining components were from the commercially available pyrene actin polymerization kit (Cytoskeleton).

Techniques: Inhibition, Fluorescence, Labeling, Negative Control